Cloning 编辑

Dr. Jeremy Allen

教育背景: 
1996年 MRC Human Genetics Unit,英国爱丁堡大学 遗传学博士学位
1991年 英国伦敦大学国王学院 生物技术理学士荣誉学位

编辑领域
基因组、遗传学、神经系统科学、发育生物学、生物技术及生物信息学领域
 
职业经历
生物学家,以神经系统科学、发育生物学以及遗传学领域见长。他在博士阶段主要研究影响肢体发育的小鼠基因突变相关的分子遗传分析。从1996年到 2004年,Dr. Allen 一直在英国剑桥Babraham 研究所神经生物学部门从事研究工作,包括DNA/RNA操纵、提纯/分离、电泳、PCR、克隆、印迹工作、文库筛选、遗传图绘制、转基因设计与构建、人工染色体工程、用于ES细胞基因打靶的载体设计与构建、细胞培养、切片技术、小鼠行为分析、小动物外科手术以及序列数据库和分析工具等生物信息工具的研究。 Dr. Allen 从2007年开始为Edanz Editing 工作,主要编辑神经系统科学和遗传学领域的文章。
 
Dr. Allen is biologist with particular experience in the fields of neuroscience, developmental biology and genetics. His PhD topic involved molecular genetic analysis of mouse mutations that affect limb development. From 1996 to 2004, Dr. Allen was a researcher at the Department of Neurobiology, The Babraham Institute, Cambridge, UK. Dr. Allen's research skills and techniques include DNA/RNA manipulation and purification/isolation, electrophoreses, PCR, cloning, all blottings, library screening, genetic mapping, design and construction of transgenes, artificial chromosome engineering, design and construction of vectors for gene targeting in ES cells, cell culture, histological techniques, mouse behavioural analysis, small animal surgery, and bioinformatic tools such as sequence databases and analysis tools. Dr. Allen began working with Edanz Editing as an editor in fields associated with neuroscience and genetics in 2007.

Dr. Neil Davies

教育背景: 
2005年 University of NSW, Australia 博士/生物技术与生物分子科学
1998年 University of NSW Kensington 学士/数学

职业经历

Dr. Davies的博士论文题为“The effect of chromatin structure and cisplatin at the human beta-globin gene cluster.”目前在澳大利亚从事助理研究员工作,此前为法国波尔多 Université Bordeaux II 大学 François Magendie Institute for Neuroscience的博士后研究人员。Dr. Davies是几家国际期刊的审稿人,编辑过大量英语母语及非母语作者的稿件。从2006年开始为Edanz Editing工作。他的编辑领域最好通过他具体的实验室工作经验来描述,包括:急性器官型培养、脑片和培养神经细胞的慢病毒操纵及转导、红藻氨酸和碘化丙啶染色、荧光共焦显微技术、活体小鼠模型、蛋白质印迹、电泳凝胶迁移率检测、寡核苷酸设计、染色体DNA提取、DNA人工/自动测序、多种PCR、细菌质粒载体克隆、超速离心分离、蛋白质三维结构计算机模拟分析以及放射物处理等。

Dr. Davies’ PhD was entitled “The effect of chromatin structure and cisplatin at the human beta-globin gene cluster.” Presently a research associate in Australia, Dr. Davies has also been a Postdoctoral Fellow at the François Magendie Institute for Neuroscience, Université Bordeaux II, Bordeaux, France. Dr. Davies is a regular reviewer for several international journals and has edited numerous manuscripts written by both native and non-native English speakers. Dr. Davies began working as an editor for Edanz Editing in 2006. His fields of editing are perhaps best described through details of his laboratory skills, which include: acute and organotypic culture, lentivirus manipulation and transduction of brain slices and cultured neuronal cells, kainate and propidium iodide staining, fluorescent and confocal microscopy, in vivo mouse models, Western blot, electrophoretic gel mobility shift assay, design of oligonucleotides, genomic DNA extraction, DNA sequencing-manual & automated, PCR (most types), cloning into bacterial plasmid vectors, ultracentrifugation, 3D protein computer modeling, radiation handling.

Dr. Christi Kuhn

教育背景: 
2003年7月 University of Regensburg, Germany 博士/神经系统科学
1995年 Colorado State University, Colorado USA 硕士/微生物学
1992年 Colorado State University, Colorado USA 学士/微生物学

职业经历
作为一名科研人员,Dr. Kuhn具有广泛的研究经验,以第一作者的身份发表了许多文章。从2007年开始为Edanz Editing工作,主要编辑神经系统科学、生理学、大脑康复/修复;出生后以及成年神经发生、记忆、学习;神经退化性疾病、干细胞;分子生物学,包括 PCR, 克隆、亚克隆、抑制消减杂交、质粒制备/纯化、cDNA, RNA和基因组DNA纯化等。此外还有相关实验室测试化验经验。
 
Dr. Kuhn has extensive experience as a research scientist and is well published as a first author. She began as an editor for Edanz Editing in 2007 and edits in fields associated with neuroscience, physiology, brain rehabilitation/repair, postnatal and adult neurogenesis, memory, learning, neurodegenerative disease, stem cells, molecular biology, including PCR, cloning, subcloning, subtractive hybridization, plasmid preparation/purification, cDNA, RNA, and genomic DNA purification. She also has working experience with all associated laboratory tests and skills necessary for that environment.

Dr. Anna Thompson

教育背景: 
1999 年 University of London (UCL), UK 博士/肾生理学
1995 年 Manchester University, UK 学士(荣誉学位)/应用生物学

职业经历

Dr. Thompson 主要致力于肾生理学/内分泌学、糖尿病和生殖学领域的科学研究。在博士研究阶段,她深入探究了类固醇代谢对肾功能的影响,并通过博士后阶段进一步研究了各种系统(包括人类胎盘和糖尿病模型鼠)中的类固醇代谢。她还研究了葡萄糖毒性对糖尿病动物模型细胞内信号传导的影响。研究技能包括:类固醇放射免疫试验,组织学,mRNA/DNA 提取和纯化,RT-PCR, 原位 RT-PCR,原代细胞培养,DNA 纯化、复制及足迹法,Northern、Southern 和 Western 免疫印迹,光学/荧光/共焦显微法等。Dr. Thompson 具有出色的写作技巧,著有大量的同行评审期刊文章,从事了五年的全职医学作者工作,写作范围涉及糖尿病学、胃肠病学、免疫学、肿瘤学和哮喘等众多领域。Dr. Thompson 从 2008 年起开始为 Edanz Editing 工作。

Dr. Thompson's scientific background is in physiology with an emphasis on renal physiology/endocrinology, diabetes and reproduction. For her PhD she investigated the effects of steroid metabolism on renal function and as a Postdoctoral Fellow she focused on steroid metabolism in various systems including human placenta and diabetic mice models. She also investigated the impact of glucose toxicity on intracellular signaling in diabetic animal models. Technical skills include steroid radio-immunoassay, histology, mRNA/DNA extraction and purification, RT-PCR, in situ RT-PCR, primary cell culture, DNA purification, cloning and footprinting, Northern, Southern and Western blots and light/fluorescence/confocal microscopy. Dr. Thompson has excellent writing skills and has written numerous peer-reviewed journal articles. She has over 5 years’ experience as a full-time Medical Writer in a wide range of topics including diabetes, gastroenterology, immunology, oncology and asthma. Dr Thompson began working with Edanz Editing in 2008.

Shara McAuley

教育背景: 
2002年 Monash University 硕士/生殖学
1999年 The University of Melbourne 学士/农业科学

职业经历
McAuley女士是大学讲师和科研人员,主要研究大型动物遗传学以及生殖内分泌学、分子生物学、妊娠与分娩、性分化和性发展以及人工繁殖技术等。她的实验室工作经验包括蛋白质定量检测(RIA, ELISA)、分子生物学技术(DNA克隆、质粒制备和纯化、电泳)、细胞培养、转染、胚胎体外成产、卵细胞采集、胚胎评级和玻璃化。在兽医领域的经验包括人工授精、胚胎移植、子宫冲洗以及尸体剖检。她于2007年加入Edanz Editing。
 
Ms. McAuley has worked as a research scientist and lecturer focusing on large animals in areas of genetics; reproductive endocrinology, molecular biology, pregnancy and parturition, sexual differentiation and development, and artificial breeding technologies. Ms. McAuley’s Laboratory Skills include protein assays (RIA, ELISA), molecular biology techniques (DNA cloning, plasmid preparation and purification, electrophoresis), cell culture, transfection, in-vitro production of embryos, oocyte aspiration, and embryo grading and vitrification. Her veterinary skills include artificial insemination, embryo transfer, uterine flushing, and necropsy. She began as an editor with Edanz Editing in 2007.

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